Preparation of Thin Sections

Routine Preparation of Potished Thin-sections

The colour plate which appears in this number of the Canadian Mineralogist iliustrates the advantage of microscopic observation of mineralized rocks in polished thin-section by incident and transmitted cross-polarized light simultaneously: the identities and relationships of opuqrr" and translucent minerals in contact are immediately evident. The principle is not ne.w; more than a hundred years...

Preparation of thin epoxy resin sections from thick sections of paraffin-embedded material.

PROCEDURES The following have been used with success: haematoxylin and eosin, Perls's reaction for ferric iron, the periodic acid-Schiff reaction for carbohydrates, the alcian blue method for acid mucopolysaccharides, the Feulgen reaction for desoxyribonucleic acid, the periodic acid-hexamine silver method, Luxol fast blue for myelin, solochrome cyanine as a general stain, Heidenhain's iron hae...

Thin Sections

Knowledge of the thickness of sections is important for proper interpretation of electron micrographs. Therefore, the thicknesses of sections of n-butyl methacrylate polymer were determined by ellipsometry, and correlated with the color shown in reflected light. The results are: gray, thinner than 60 mmicro; silver, 60 to 90 mmicro; gold, 90 to 150 mmicro; purple, 150 to 190 mmicro; blue, 190 t...

Preparation of Tissue Sections

A simple reproducible protocol for detecting multiple copy human genes and viral DNA in routine formalin fixed paraffin embedded tonsil and brain, by in situ hybridisation with biotinylated probes, is described. The protocol consists of digestion of formalin fixed paraffin sections, with 0 4% pepsin in 0 01 M hydrochloric acid for one hour at 37°C, followed by hybridisation with biotinylated pr...

Educational Meteorite Thin Sections